Oilgonucleotide Modifications

Some of the Available Modifications

Type of modification	Price per insertion, 100 or 200 nmole scale*	Price per insertion, 1000 nmole scale
Degenerated bases (equimolar mix)	$0.00	$0.00
Degenerated bases (non-equimolar mix, also known as "doping")	US$2 / CAN$3	US$4 / CAN$6
5' Acrydite	US$40 / CAN$60	US$80 / CAN$120
Inosine (deoxyinosine, dI)	US$6.67 / CAN$10	US$13 / CAN$20
Uridine (deoxyuridine, dU)	US$6.67 / CAN$10	US$13 / CAN$20
3' Phosphorylation	US$15 / CAN$23	US$30 / CAN$45
5' Phosphorylation	US$24 / CAN$35	US$48 / CAN$70
3' Amine	US$15 /CAN$23	US$30 / CAN$45
5' Amine - C6	US$24 / CAN$35	US$48 / CAN$75
3' Thiol	US$25 / CAN$37	US$50 / CAN$75
5' Thiol	US$40 / CAN$60	US$80 / CAN$120
3' Biotin Labeling	US$25 / CAN$37	US$50 / CAN$75
5' Biotin Labeling	US$39 / CAN$60	US$78 / CAN$120
3' Digoxigenin	US$120 / CAN$180	US$240 / CAN$360
5' Digoxigenin	US$120 / CAN$180	US$240 / CAN$360
3'-end 6-FAM, HEX or TET	US$49 / CAN$74	US$149 / CAN$223
5'-end 6-FAM, HEX or TET	US$29 / CAN$45	US$75 / CAN$120
3' BHQ-1(black hole quencher)	US$50 / CAN$75	US$100 / CAN$150
3' BHQ-2	US$50 / CAN$75	US$100 / CAN$150
3' BHQ-3	US$50 / CAN$75	US$100 / CAN$150
3' Dabcyl	US$49 / CAN$74	US$99 / CAN$149
5' TAMRA (may replace NED)	US$80 / CAN$120	US$180 / CAN$270
3' TAMRA	US$49 / CAN$74	US$149 / CAN$223
5' Fluorescein (FITC)	US$24 / CAN$36	US$50 / CAN$75
3' Fluorescein Labeling	US$25 / CAN$37	US$50 / CAN$75
3' Cy3 dye	US$80 / CAN$100	US$150 / CAN$200
5' Cy3 dye	US$80 / CAN$100	US$150 / CAN$200
3' Cy5 dye	US$80 / CAN$100	US$150 / CAN$200
5' Cy5 dye (may replace D4)	US$80 / CAN$100	US$150 / CAN$200
5' Cy5.5 dye (may replace D3)	US$160 / CAN$200	US$300 / CAN$400
5' Texas Red (may replace PET, ROX or Cy3.5)	US$120 / CAN$150 (US$67 / CAN$100 for 4th and consecituve dye per order)	US$200 / CAN$250
Yakima Yellow (may replace VIC)	US$200 / CAN$250 (US$100 / CAN$120 for 4th and consecituve dye per order)	US$400 / CAN$500
2'-O-Me RNA	US$4 / CAN$6	US$8 / CAN$12
Other modifications	Please inquire	Please inquire
Discounts for more than one oligo from the same type of modification	Please inquire	Please inquire

*200 nmole (0.2 micromole) scale synthesis will yield typically 0.1-0.2 micromole (100-200 nmole) final product. Guaranteed minimum 50 nmole for regular size (up to 25-mer) oligos, standard purity (desalted). For longer oligos, 10 OD260 guaranteed minimum (standard purity). Standard purity includes free desalting. All oligos are quantified and three different units of measure are provided to the customer. The exact relation between these three units is calculated by a computer for each oligo; as a very rough approximation for a 20 base long oligo, 100 nmole equals about 20 OD260 units, or 600 microgram. Example of price calculation for one oligonucleotide, 20-mer, 100 nmole scale, 5'-end labeled with 6-FAM: 20 x 0.29 = 5.80 basic price of the oligo, plus 29.00 for the 6-FAM, total = 34.80 USD.

Phosphorothioated oligonucleotides (PTO)

Synthesis scale	Additional charge per PTO base	Basic cost per base	Setup charge
0.2 umole	US$1.50** / CAN$2.00 per PTO base	US$0.48** / CAN$0.74	$0.00
1.0 umole	US$1.50 / CAN$2.23 per PTO base	US$1.50 / CAN$2.23	$0.00
15 umole	US$10.00 / CAN$15.00 per PTO base	US$6.50 / CAN$10.00	US$30 / CAN$50

**Example: One oligo of 20 bases at the 200 nmole scale, of which 3 bases at each end are phosphorothioated: 20 x 0.48 = 9.60, plus (3+3) x 1.50 = 9.00, for the total of 18.60.